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Part:BBa_K4829006:Design

Designed by: Aditya Kamath Ammembal   Group: iGEM23_IISc-Bengaluru   (2023-10-04)


PolyA tail for an mRNA sequence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A poly-A tail CANNOT be synthesised in gene fragments, a difficulty we had to deal with. We ended up synthesising the mRNA without the tail and then tailing with PolyA polymerase, as we used a PCR product. However, if the plasmid has a tail already in it, that's great!

Source

Not from any genome specifically.

References