Other
Part:BBa_K4829006:Design
Designed by: Aditya Kamath Ammembal Group: iGEM23_IISc-Bengaluru (2023-10-04)
PolyA tail for an mRNA sequence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A poly-A tail CANNOT be synthesised in gene fragments, a difficulty we had to deal with. We ended up synthesising the mRNA without the tail and then tailing with PolyA polymerase, as we used a PCR product. However, if the plasmid has a tail already in it, that's great!
Source
Not from any genome specifically.